After finishing a section and not using the rest of the tissue in the water bath, what should be done to prevent carryover?

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Multiple Choice

After finishing a section and not using the rest of the tissue in the water bath, what should be done to prevent carryover?

Carryover happens when tissue fragments from one section float in the water bath and are picked up by the next section. If debris remains, those fragments can contaminate subsequent samples and affect staining or interpretation. The best action is to remove all debris from the bath so the next section starts with clean water. In practice, lift out debris with forceps, rinse or replace the water bath, and ensure it’s clean before continuing. Leaving debris in the bath would cause carryover; simply changing temperature won’t remove debris; adding more tissue would worsen the problem.

Carryover happens when tissue fragments from one section float in the water bath and are picked up by the next section. If debris remains, those fragments can contaminate subsequent samples and affect staining or interpretation. The best action is to remove all debris from the bath so the next section starts with clean water. In practice, lift out debris with forceps, rinse or replace the water bath, and ensure it’s clean before continuing. Leaving debris in the bath would cause carryover; simply changing temperature won’t remove debris; adding more tissue would worsen the problem.

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