If bubbles appear during Oil Red O staining, what is the recommended remedy?

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Oil Red O stains lipids by immersing tissue in a lipid-loving dye solvent. When bubbles appear, they’re usually air pockets that prevent the dye from contacting lipid droplets evenly. Soaking the slide in warm water helps release trapped air and allows the stain to penetrate more uniformly, reducing those bubbles and giving a more consistent red staining of lipid areas. Warming also helps rehydrate the tissue slightly and facilitates better diffusion of the dye. The other options wouldn’t fix the air pockets: more stain or longer staining won’t guarantee contact where air is present, and using a resinous mounting medium isn’t compatible with lipid preservation and wouldn’t resolve bubbles formed during staining.