In Gomori's reticulin stain, which is the first step in the sequence?

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Multiple Choice

In Gomori's reticulin stain, which is the first step in the sequence?

In Gomori's reticulin stain, the first step is oxidation with potassium permanganate. This oxidation activates tissue components, creating reactive sites (such as aldehyde groups) that set up the tissue to bind silver in the subsequent steps. After this, ferric ammonium sulfate sensitizes those sites, formalin acts as the reducer to deposit metallic silver on the prepared areas, and sodium thiosulfate fixes by removing unbound silver. This sequence explains why oxidation is done first—the preparatory step that enables the silver impregnation of reticular fibers.

In Gomori's reticulin stain, the first step is oxidation with potassium permanganate. This oxidation activates tissue components, creating reactive sites (such as aldehyde groups) that set up the tissue to bind silver in the subsequent steps. After this, ferric ammonium sulfate sensitizes those sites, formalin acts as the reducer to deposit metallic silver on the prepared areas, and sodium thiosulfate fixes by removing unbound silver. This sequence explains why oxidation is done first—the preparatory step that enables the silver impregnation of reticular fibers.

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