In Masson Trichrome, which reagent stains collagen?

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Multiple Choice

In Masson Trichrome, which reagent stains collagen?

Masson’s trichrome uses a combination of dyes to differentiate tissue components, with collagen distinguished by a green color. After the nuclear stain and the red-staining step for cytoplasm/muscle, the collagen fibers take up the green dye (light green) during the color-separation step, yielding green-stained collagen. Bouin’s solution acts as a mordant/fixative to boost contrast, while the iron hematoxylin stains nuclei dark and the red stain highlights cytoplasm and muscle. So, the reagent that marks collagen is the light green dye, giving collagen its green coloration in this protocol.

Masson’s trichrome uses a combination of dyes to differentiate tissue components, with collagen distinguished by a green color. After the nuclear stain and the red-staining step for cytoplasm/muscle, the collagen fibers take up the green dye (light green) during the color-separation step, yielding green-stained collagen. Bouin’s solution acts as a mordant/fixative to boost contrast, while the iron hematoxylin stains nuclei dark and the red stain highlights cytoplasm and muscle. So, the reagent that marks collagen is the light green dye, giving collagen its green coloration in this protocol.

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