In PAS staining, what is the purpose of diastase when glycogen is being demonstrated?

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Multiple Choice

In PAS staining, what is the purpose of diastase when glycogen is being demonstrated?

Diastase digestion is used to confirm glycogen by removing it from the tissue. Glycogen is PAS-positive, so after treating with diastase, the areas containing glycogen lose their PAS stain. If the staining disappears, glycogen was present; if staining remains, the PAS positivity comes from other diastase-resistant carbohydrates such as certain mucopolysaccharides or glycoproteins.

Diastase digestion is used to confirm glycogen by removing it from the tissue. Glycogen is PAS-positive, so after treating with diastase, the areas containing glycogen lose their PAS stain. If the staining disappears, glycogen was present; if staining remains, the PAS positivity comes from other diastase-resistant carbohydrates such as certain mucopolysaccharides or glycoproteins.

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