What is the second step of tissue processing and what is the purpose?

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Multiple Choice

What is the second step of tissue processing and what is the purpose?

Clearing is the step that follows dehydration. Its purpose is to remove the dehydrating solvent (the alcohol) and replace it with a clearing agent such as xylene, which is miscible with paraffin. This replacement makes the tissue optically transparent and prepares it for infiltration with paraffin, so it can be embedded for sectioning.

Clearing is the step that follows dehydration. Its purpose is to remove the dehydrating solvent (the alcohol) and replace it with a clearing agent such as xylene, which is miscible with paraffin. This replacement makes the tissue optically transparent and prepares it for infiltration with paraffin, so it can be embedded for sectioning.

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