Study for the CAMLPR Histology Practice Test. Study with flashcards and multiple choice questions, each question has hints and explanations. Get ready for your exam!

Multiple Choice

What is the sequence after dehydration in standard tissue processing?

After dehydration, the tissue is cleared with a clearing agent, which replaces the alcohol and makes the tissue compatible with paraffin wax. This is followed by infiltration with molten paraffin wax, which permeates the tissue and enables embedding for sectioning. Clearing agents like xylene dissolve residual alcohol and render the tissue transparent, preparing it to mix with the hydrocarbon paraffin. Embedding before clearing isn’t feasible because paraffin infiltration depends on removing alcohol and replacing it with a medium that can be displaced by paraffin. Staining is done after sections are prepared, not during the block-processing steps. Rehydration after dehydration would undo the dehydration and is not part of the standard sequence for paraffin embedding.

After dehydration, the tissue is cleared with a clearing agent, which replaces the alcohol and makes the tissue compatible with paraffin wax. This is followed by infiltration with molten paraffin wax, which permeates the tissue and enables embedding for sectioning. Clearing agents like xylene dissolve residual alcohol and render the tissue transparent, preparing it to mix with the hydrocarbon paraffin. Embedding before clearing isn’t feasible because paraffin infiltration depends on removing alcohol and replacing it with a medium that can be displaced by paraffin. Staining is done after sections are prepared, not during the block-processing steps. Rehydration after dehydration would undo the dehydration and is not part of the standard sequence for paraffin embedding.