Which sequence correctly lists the components and sequence of the Verhoeff's Van Gieson method?

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Multiple Choice

Which sequence correctly lists the components and sequence of the Verhoeff's Van Gieson method?

The sequence tested is the correct order of staining steps to visualize elastic fibers and collagen distinctly in the Verhoeff's Van Gieson method. You begin by applying Verhoeff's solution to stain elastic fibers, which gives them a dark color (typically black). Next, you differentiate with ferric chloride to remove non-elastic elements and sharpen the elastic-stain contrast. After differentiation, you wash and then treat with 95% alcohol to remove excess iodine and any residual stain, preparing the tissue for the counterstain. Finally, you apply Van Gieson, which colors collagen pink/red and other tissue yellow, providing clear contrast against the elastin. This order is essential to achieve the desired differential staining: elastin stands out dark while collagen and other tissues take on the counterstain colors. Reversing the sequence or misplacing steps—such as counterstaining before staining elastin, or applying alcohol before differentiation—would blur or miscolor the components and defeat the purpose of the method.

The sequence tested is the correct order of staining steps to visualize elastic fibers and collagen distinctly in the Verhoeff's Van Gieson method. You begin by applying Verhoeff's solution to stain elastic fibers, which gives them a dark color (typically black). Next, you differentiate with ferric chloride to remove non-elastic elements and sharpen the elastic-stain contrast. After differentiation, you wash and then treat with 95% alcohol to remove excess iodine and any residual stain, preparing the tissue for the counterstain. Finally, you apply Van Gieson, which colors collagen pink/red and other tissue yellow, providing clear contrast against the elastin.

This order is essential to achieve the desired differential staining: elastin stands out dark while collagen and other tissues take on the counterstain colors. Reversing the sequence or misplacing steps—such as counterstaining before staining elastin, or applying alcohol before differentiation—would blur or miscolor the components and defeat the purpose of the method.

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