Which stain protocol uses Harris hematoxylin as the nuclear stain?

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Multiple Choice

Which stain protocol uses Harris hematoxylin as the nuclear stain?

Congo red staining for amyloid uses the dye to bind the amyloid deposits, and a nuclear counterstain is added to provide contrast against the stained tissue. Harris hematoxylin is a common choice for that counterstain, coloring nuclei blue and allowing the amyloid deposits stained by Congo red to stand out. Additionally, under polarized light Congo red–stained amyloid shows the characteristic apple-green birefringence, which helps confirm the diagnosis. The other stains listed use different nuclear counterstains or are not typically paired with Congo red, so Harris hematoxylin is most closely associated with the Congo red protocol.

Congo red staining for amyloid uses the dye to bind the amyloid deposits, and a nuclear counterstain is added to provide contrast against the stained tissue. Harris hematoxylin is a common choice for that counterstain, coloring nuclei blue and allowing the amyloid deposits stained by Congo red to stand out. Additionally, under polarized light Congo red–stained amyloid shows the characteristic apple-green birefringence, which helps confirm the diagnosis. The other stains listed use different nuclear counterstains or are not typically paired with Congo red, so Harris hematoxylin is most closely associated with the Congo red protocol.

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