Which stain uses Metanil Yellow as a counterstain?

Study for the CAMLPR Histology Practice Test. Study with flashcards and multiple choice questions, each question has hints and explanations. Get ready for your exam!

Multiple Choice

Which stain uses Metanil Yellow as a counterstain?

Metanil Yellow serves as a counterstain in Mayer's mucicarmine staining to create a strong contrast that makes mucin stand out. In this method, the primary stain Carmine binds to mucin and colors it magenta, while Metanil Yellow stains the surrounding tissue yellow. This combination is characteristic of mucicarmine and is particularly useful for highlighting mucin in goblet cells and mucin-producing tissues. Other stains don’t use this yellow counterstain: Alcian blue targets acidic mucopolysaccharides and appears blue, Congo Red highlights amyloid, and Osmium tetroxide is used for lipid staining and fixation, not paired with Metanil Yellow in this context.

Metanil Yellow serves as a counterstain in Mayer's mucicarmine staining to create a strong contrast that makes mucin stand out. In this method, the primary stain Carmine binds to mucin and colors it magenta, while Metanil Yellow stains the surrounding tissue yellow. This combination is characteristic of mucicarmine and is particularly useful for highlighting mucin in goblet cells and mucin-producing tissues.

Other stains don’t use this yellow counterstain: Alcian blue targets acidic mucopolysaccharides and appears blue, Congo Red highlights amyloid, and Osmium tetroxide is used for lipid staining and fixation, not paired with Metanil Yellow in this context.

More practice questions